BCL2L1 Knockout cell line (HEL)

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BCL2L1 Knockout cell line (HEL) Order

BCL2L1 Knockout cell line (HEL)
Catalog#
YKO-KP178
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
BCL2L1 Knockout cell line (HEL)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
BCL2L1 Knockout cell line (HEL)
Cell Morphology
Lymphoblast,suspension
Passage ratio
1：2-1：4
Species
Human
Gene Name
BCL2L1
Gene ID
598
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Accession
CVCL_D9W4(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript BCL2L1-201)
Validation: PCR+Sanger Sequencing
BCL2L1 Knockout cell line (HEL) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HEL		 STR Info (Cell bank)
Cell Line:HEL
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 911911
D7S820 7777
D16S539 11111111
VWA 14171417
TH01 7777
AMEL XXXX
TPOX 11111111
CSF1PO 10101010
D12S391 1821
FGA 212223.0
D2S1338 1819
D21S11 2930.231.2
D18S51 1216
D8S1179 1315
D3S1358 1515
D6S1043 1113
PENTAE 1318
D19S433 1113
PENTAD 1113
D1S1656 17.317.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BCL2L1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEL cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BCL2L1 gene knockout BCL2L1 Knockout cell line (HEL) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
BCL2L1 Knockout cell line (HEL)
BCL2L1 Knockout cell line (HEL)
Catalog#
YKO-KP178
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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